Kendricklabs | Protein Analysis Services

When it comes to analyzing proteins, one technique stands out as a cornerstone in the field of biochemistry: SDS-PAGE electrophoresis. Developed by Kendrick Labs Inc., this powerful method has been instrumental in our understanding of proteins and their various functions. In this article, we will explore the fundamentals of SDS-PAGE electrophoresis, its applications, and how it has revolutionized the world of molecular biology.   The Basics of SDS-PAGE Electrophoresis SDS-PAGE, or Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, is a widely used technique for separating proteins based on their molecular weight. It relies on the principles of electrophoresis to drive the movement of charged molecules through a gel matrix. Here's how it works:   Sample Preparation: First, the protein sample is prepared by denaturing the proteins using a detergent called SDS (Sodium Dodecyl Sulfate). This process unfolds the proteins and coats them with a negative charge, maki...

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique in biochemistry and molecular biology laboratories for separating and analyzing proteins based on their size. While SDS-PAGE is a powerful tool with numerous advantages, it also comes with several limitations and challenges that researchers need to be aware of. In this article, provided by Kendrick Labs, we will explore these limitations and challenges in detail and discuss strategies to overcome them. Limited Separation Based on Size SDS-PAGE is primarily a size-based separation technique, where proteins are separated based on their molecular weight. While this is advantageous for many applications, it is also a limitation because proteins with similar molecular weights may not be effectively resolved. Additionally, very large proteins or protein complexes may not enter the gel matrix or migrate well during electrophoresis. Solution: For resolving proteins with similar molecular wei...