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Showing posts from March, 2025

SDS-PAGE for Detecting Protein Contamination in Manufacturing

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 In pharmaceutical, biotech, and industrial manufacturing, maintaining protein purity is critical to ensuring the quality, efficacy, and safety of final products. Protein contamination can compromise research results, disrupt production processes, and even pose risks to patients and consumers. SDS PAGE Electrophoresis is a gold-standard technique widely used for detecting protein contamination at various stages of production. It provides a reliable, high-resolution method to analyze protein composition and ensure quality control. Why Protein Contamination is a Concern in Manufacturing Protein-based drugs, vaccines, and diagnostic products require stringent quality control to prevent contamination that could lead to unwanted immunogenic responses or reduced efficacy. Even minor contamination can impact batch consistency, making detection methods essential. SDS PAGE Electrophoresis plays a pivotal role in assessing protein purity and identifying unwanted protein contaminants in b...

Streamlined Strategies for Immunoblot & Western Blot: Data Tips!

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Western blotting and immunoblot techniques are essential tools in protein analysis for pharmaceutical, academic, and industrial research. Ensuring accurate, reproducible, and high-quality results requires streamlined strategies that optimize efficiency while minimizing errors. At Kendrick Labs, Inc., we specialize in providing professional lab services to support precise protein analysis. This guide offers essential tips for enhancing the Immunoblot Western Blot workflow. Optimizing Sample Preparation Protein Extraction ·          Use lysis buffers appropriate for your target proteins to ensure efficient extraction without degradation. ·          Include protease and phosphatase inhibitors to prevent protein degradation during extraction. ·          Optimize sonication or mechanical disruption based on the sample type to enhance protein yield. Protein Quant...