How to Measure Protein Concentration SDS PAGE for Accurate Results?
As someone who has spent years in the lab, one of the most critical tasks I’ve learned to master is the measurement of protein concentration using SDS PAGE. It might seem routine at first, but ensuring accuracy can truly make or break your experiments. In this post, I’ll share a detailed walkthrough of how I measure protein concentration with SDS PAGE, offering practical tips and real-world advice to help you get reliable and reproducible results every time. Understanding the Basics of SDS PAGE SDS PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is a widely used technique in biochemistry and molecular biology to separate proteins based on their molecular weight. SDS, an anionic detergent, denatures proteins and imparts a negative charge proportional to their length. When subjected to an electric field, these proteins migrate through a polyacrylamide gel matrix, allowing us to visualize and quantify them. At Kendrick Labs, Inc, a trusted name in protein analysis s...