Kendricklabs | Protein Analysis Services

Working hands-on with complex protein samples has taught me one important lesson: meaningful protein analysis requires precision, patience, and the right separation tools. Over the years, 2D gel electrophoresis has become one of my most trusted techniques for resolving complex proteomes. When the goal is to visualize protein expression patterns clearly and detect subtle molecular differences, this method continues to deliver unmatched clarity. In this article, I share my direct experience using 2D gel electrophoresis for advanced protein separation analysis, how I apply it in real research scenarios, and why it still holds strong value in modern proteomics. Why I Continue to Use 2D Gel Electrophoresis Protein mixtures are inherently complex. In my work, I often encounter samples containing thousands of proteins with overlapping sizes, charges, and modifications. Single-dimension separation methods rarely provide the resolution I need. That’s why I rely on 2D gel electrophoresis—i...